Optimization of alloxan dose is essential to induce stable. Diabetes was induced in albino rats by single intraperitoneal injection of alloxan 70mgkg body weight. However, as in alloxan treated, fatfed swine, superimposition of stz induced diabetes on hyperlipemia results in a significant increase, similar to that seen in human diabetic subjects, in plasma triglycerides. Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. Studies of type 2 dm models should provide further understanding of the underlying mechanisms of diabetic atherosclerosis. Alloxaninduced diabetes, a common model for evaluating the. In the present study, we report the effects of induced diabetes and its treatment with or without oral hypoglycaemic drugs on the morphology and oxidative stress status of the prefrontal cortex. Alloxan induced diabetes is a model of type 1 dm, but type 2 dm is a more common risk factor for cvd. Our results showed that daily supplementation of vco 20% of food for 16 weeks signifi. Research article alloxan induced diabetes mellitus in wistar. Jun 26, 2012 in fact, the administration of glibenclamide inhibited all changes caused by alloxan induced diabetes. We report herein the findings of this research work.
Diabetes follows the alloxaninduced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet. W m vogel and c s apstein houseman medical research center, department of pharmacology, boston university school of medicine, ma 021182394. Some antidiabetic properties of prosopis farcta extracts in. Cuiidependent dna damage increased with the concentration of alloxan and nadh. Alloxan induced diabetes, a common model for evaluating the glycemiccontrol potential of therapeutic compounds and plants extracts in experimental studies medicina, vol. Effects of alloxaninduced diabetes on ischemiareperfusion. The resulting insulinopenia causes a state of experimental diabetes mellitus called alloxan diabetes 4, 8, 9. Antidiabetic activity of bauhinia forficata extracts in. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Antihyperglycemic effects of fermented and nonfermented mung. Alloxan has been administered in single or multiple. They found that alloxan has a specific necrosing action on both the pancreatic islet cells and the epithelium of the renal convoluted tubules when injected into rabbits intravenously or intraperitoneally in doses of 100 to 500 mg.
Two other groups induced with diabetes using alloxan by a single dose administration of 120 mgkg body weight bw. Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal. The mechanisms of alloxan and streptozotocininduced. Hyperglycaemia was induced in fasted wistar rats with alloxan 150 mgkg body weight. The effect of chronic alloxan and streptozotocininduced. This study was undertaken to investigation the effect of diasulin, a poly herbal drug composed of ethanolic extract of ten medicinal plants on blood glucose, plasma insulin, tissue lipid profile, and lipidperoxidation in alloxan induced diabetes. The inhibition of the steroids induced no synthesis by using name ngmethyllarginine acetate ester in the reaction mixture resulted in the inhibition of hepatic insulin synthesis. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l.
This causes an insulindependent diabetes mellitus called alloxan diabetes in these animals, with characteristics similar to type 1 diabetes in humans lenzen, 2008. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. Glucokinase gk is hypothesized to be the critical glucosensor of pancreatic. Experimental diabetes induced by alloxan and streptozotocin. Alloxaninduced diabetes causes morphological and ultrastructural. To understand the role of gk in glucoprivic counterregulatory responses, we injected alloxan, a gk inhibitor and toxin, into the third ventricle 3v to target nearby gkexpressing neurons. Pdf estimation of glucose level and body weight in.
The leaf essential oil of citrus sinensis was administered every other day to the diabetic rats for a period of 15 days. Alloxan is known to induce diabetes in experimental animals through destruction of insulinproducing 3cells of pancreas. Treatment of alloxaninduced diabetic rats with metformin or. Diabetes was induced in wistar albino rats by intraperitoneal injection of alloxan 120 mgkg bw once.
Ever since streptozotocin has been the agent of choice to induce experimental diabetes mellitus in lab oratory animals rerup, 1970. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. Pdf alloxaninduced diabetes, a common model for evaluating the. The beta cell toxic action of alloxan is initiated by free radicals formed in this redox reaction. Raw camel milk properties on alloxaninduced diabetic wistar rats. The effect of chronic alloxan and streptozotocininduced diabetes on isolated rat heart performance. Three days later diabetes was confirmed using one touch glucometer. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Alloxaninduced diabetes, a common model for evaluating the glycemiccontrol potential of therapeutic compounds and plants extracts in experimental studies medicina, vol.
Alloxan dose of 120, 140, 160 and 180 mg kg 1 body weight was given intraperitonealy to experimental rats in groups of 6 animals for each dose. The control of hyperglycemia by estriol and progesterone in. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Diabetes was induced in the animals by intraperitoneal administration of single dose alloxan monohydrate 150 mgkg body weight. Diabetes was induced using intraperitoneal injection of alloxan 100 mgkg, 0. As shown in figure 4 b, vacuolated hepatocytes with the nucleus being pushed to the periphery and fatty cyst were observed in diabetic rats. Alloxan induced rats,anti diabetes, diabetes, ipomoea batatas, phytochemical analysis. Antihyperglycemic effect of gasca d herbal formulation on alloxan. Alloxaninduced diabetes mellitus how is alloxaninduced. Dawley rats with diabetes mellitus induced by alloxan dmia rats and streptozotocin dmis rats, the protein expression and. Diabetes mellitus is a metabolic disorder characterized by disturbances in the metabolism of carbohydrates, proteins and lipids as a result of absolute or moderate. Introduction diabetes mellitus diabetes is a group of metabolic diseases in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is.
The aim of our study was to evaluate the antidiabetic activity of camel milk on serum glucose and lipid profile of alloxaninduced diabetic rats. Antioxidant and prebiotic effects of a beverage composed. Moringa oleifera leaf extract ameliorates alloxaninduced. B activation and protects against alloxaninduced diabetes in cd1 mice. The root extract was the best one for reduction of. Alloxaninduced diabetes is a model of type 1 dm, but type 2 dm is a more common risk factor for cvd.
Although there was no effect of diabetes on the cholesterol content of grossly normal thoracic and abdominal aortic intima, abdominal. Studies on the administration of vernonia amygdalina del. Alloxan definition is a crystalline compound c4h2n2o4 causing diabetes mellitus when injected into experimental animals. Brazilian morus nigra attenuated hyperglycemia, dyslipidemia. Normal diet supplemented with 10% and 20% of kernel cake of balanites aegyptiaca were fed to diabetic rats for three weeks. Alloxaninduced diabetes, a common model for evaluating. Essential oils samples were obtained from the aerial parts of the plant by hydrodistillation and analyzed by gcms. Alloxan is a toxic glucose analogue, which selectively destroys insulinproducing cells in the pancreas when administered to rodents and many other animal species. Altered glucose metabolism and hypoxic response in alloxan. This study was undertaken to investigate the preventive mechanism of amomum xanthoides extract against the development of alloxan induced diabetics of mice. This study was undertaken to investigate the preventive mechanism of amomum xanthoides extract against the development of alloxaninduced diabetics of mice. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter.
Diabetes was induced by interperitoneal injection of alloxan monohydrate 150 mgkg bw followed by a. The protective effect of amomum xanthoides extract against. Dec 18, 2007 alloxan has two distinct pathological effects. The mechanism of alloxan and streptozotocin action in b cells. Morus nigra has been used popularly for several proposes, including diabetic. Moringa oleifera leaf extract ameliorates alloxaninduced diabetes in rats by regeneration of. Alloxan definition of alloxan by medical dictionary. Neurobehavioural and cognitive impairments are reportedly associated with both types of diabetes mellitus. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. Ethanolic extract of diasulin a, poly herbal drug was administered orally 200 mgkg body weight for 30 days. Antihyperlipidemic and antiperoxidative effect of diasulin, a. The root extract was the best one for reduction of serum glucose followed by leaves then fruits. The present study described the phytochemical profile of lavandula stoechas essential oils, collected in the area of aindraham northwest of tunisia, as well as their protective effects against alloxaninduced diabetes and oxidative stress in rat.
Albino rats each weighing 180230g were divided into 3 equal groups n10. The mechanism of dna damage induced by alloxan was investigated using 32plabeled human dna fragments. Treatments of the alloxaninduced diabetes rats were done by daily oral administration of different concentrations with p. Furthermore, in the present study, the treatment of alloxaninduced hyperglycaemic rats with oral hypoglycaemic drugs metformin, pioglitazone and rosiglitazone protected the. Induction of stable diabetes for prolonged period is needed for studies on various parameters in diabetes. Treatments of the alloxan induced diabetes rats were done by daily oral administration of different concentrations with p. The aim of this study was to investigate the potential protective effect of virgin coconut oil vco on oxidative stress parameters in the liver, kidneys and heart of alloxaninduced 150 mg kg. Alloxan diabetes definition of alloxan diabetes by medical. Third ventricular alloxan reversibly impairs glucose. Studies suggests that alloxan does not cause diabetes in humans. The protective role of virgin coconut oil on the alloxan.
Antidiabetic effect of ipomoea batatas in normal and alloxan. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. In fact, the administration of glibenclamide inhibited all changes caused by alloxaninduced diabetes. The hyperglycemic mice were maintained on 5% glucose solution for the next 24 h to prevent hypoglycemia and monitored for 3 days to ensure constant blood glucose levels before they were subjected to 18 hours of fasting. Pdf experimental diabetes induced by alloxan and streptozotocin. Effects of diabetes mellitus induced by alloxan on the. Diabetesinduced accelerated atherosclerosis in swine. Open access treatment of alloxaninduced diabetic rats with. Extracts were administered daily for 7d at doses of 200 and 400mgkg, p. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus. Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal effects on other structures.
The mechanisms of alloxan and streptozotocininduced diabetes. The responses are accompanied by corresponding inverse changes in. Hearts from rabbits with 816 weeks of alloxandiabetes were compared with hearts from normal rabbits to determine whether diabetic myocardium is more. Glucose prevents the development of diabetes induced by alloxan.
In the present study, the protective mechanism of glucose against alloxaninduced. Hypoglycemic and antioxidant effects of leaf essential oil of. Thereafter, several authors have used alloxaninduced diabetes model as a. The mechanism of alloxan and streptozotocin action in b. Hearts from rabbits with 816 weeks of alloxan diabetes were compared with hearts from normal rabbits to determine whether diabetic myocardium is more. Induction of diabetes diabetes mellitus was induced by intraperitoneal injection of 150 mgkg body weight of alloxan monohydrate, suspended in distilled water. Protective mechanism of glucose against alloxaninduced. Four and 6 days after 3v, but not 4v, alloxan injection, alloxantreated rats. It has been successfully induced in a variety of animal species. However, as in alloxantreated, fatfed swine, superimposition of stzinduced diabetes on hyperlipemia results in a significant increase, similar to that seen in human diabetic subjects, in plasma triglycerides. Alloxaninduced rats,antidiabetes, diabetes, ipomoea batatas, phytochemical analysis. Others found a significant difference in alloxan plasma levels in children with and without diabetes type 1.
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